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Journal of International Translational Medicine
Journal of International Translational Medicine
JITM, ISSN 2227-6394
founded in 2012
Editor-in-chief:Feng Jifeng, China
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2014 Vol. 2, No. 2
Published: 30 June 2014

 
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Anti-tumor Effect and Potential Mechanism of Arsenic Trioxide on SMMC-7721 Cells of Human Liver Cancer in Vivo and Vitro Hot!

Shen Bo
Journal of International Translational Medicine, 2014, 2(2): 288-293
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Objective: To observe the anti-tumor effect of arsenic trioxide (As2O3) on SMMC-7721 cells of patients with liver cancer in vivo and vitro. Methods: Methyl Thiazolyl Tetrazolium (MTT) method, fluorescence microscope, FITCAnnexin Ⅴ /PI double-tagging method and colorimetry were used to detect the survival rate, morphological change, apoptosis and Caspase-3 activity change of SMMC-7721 cells in different concentration of As2O3 for 48 h, respectively. As2O3 was injected into the transplanted tumors in nude mice with SMMC-7721 hepatoma cells to observe the growth of tumors. The mice were sacrificed after 12 d, followed by the extirpation and weighing of the tumors. Then Bcl-2, Bax and Caspase-3 expression were detected by immunohistochemistry. Results: As2O3 could obviously inhabit the proliferation of SMMC-7721 cells, with inhibition concentration (IC) being (18.17±2.10) μmol/L. Microscope showed typical morphological change of cell apoptosis and As2O3 treatment group was evidently higher than control group in apoptosis rate of SMMC-772 cells, suggesting that As2O3 could improve SMMC-772 cell activity. Internal injection of As2O3 into transplanted tumors in nude mice with SMMC-7721 hepatocellular lines could remarkably inhabit the growth of tumors with inhabiting rate being 52.37%, and immunohistochemistry also revealed that As2O3 could apparently up-regulate cell apoptosis-related Bax and Caspase-3 expression, and down-regulate Bcl-2 expression. Conclusion: As2O3 can inhabit the growth and in vivo oncogenesis of hepatoma SMMC-7721 cells, and induce SMMC-7721 cell apoptosis.
       

Antilipemic Effect of Berberine Combined with Atorvastatin on Rats with Hypelipemia Hot!

Lu Ye, Jiang Wenlong, Cui Junyou
Journal of International Translational Medicine, 2014, 2(2): 294-298
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Objective: To investigate the antilipemic effect of berberine combined with atorvastatin on rats with hypelipemia. Methods: The hypelipemia model was established by intragastric administration of lipid emulsion in rats. According to the level of total cholesterol (TC), the rats were randomly divided into 6 groups: model group, atorvastatin group, high-, middle- and low-dose berberine groups, and berberine + atorvastatin group. The antilipemic effect of each group was observed after 21 days, and the expressions of proprotein convertase subtilisin/kexin type 9 (PCSK9) and low density lipoprotein receptor (LDL-R) were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and West blot methods. Results: TC level was significantly decreased in atorvastatin group, high-dose berberine group and berberine + atorvastatin group, and that of low density lipoprotein (LDL) in atorvastatin group and berberine + atorvastatin group was also reduced notably. However, the decreased effects of TC and LDL in berberine + torvastatin group were superior to atorvastatin group. Both RTPCR and West blot detection results revealed that PCSK9 expression went down markedly, while LDL-R expression up in the liver of rats in berberine + torvastatin group. Conclusion: Berberine exerts an antilipemic effect by inhibiting PCSK9 and increasing LDL-R, which can be reinforced by combination with atorvastatin.
       

Mechanism of Shenyi Capsule Concomitant with Endostar and Chemotherapy on the Growth and Apoptosis of MCF-7 Breast Cancer Cells Hot!

Shao Mingwen, Qiu Jinrong, Wu Hao, Liu Yiqian, Liu Lianke
Journal of International Translational Medicine, 2014, 2(2): 299-302
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Objective: To observe the effect of Shenyi Capsule concomitant with Endosatr and chemotherapy in improving the proliferative activity and apoptosis of MCF-7 breast cancer cells. Methods: The cultured cells were randomly divided into control group, 5-Fu group, combination group 1, combination group 2 and combination group 3. The morphological changes were observed under inverted microscope, activity inhibition rate was calculated by methyl thiazolyl tetrazolium (MTT) method and fluorescence microscope was applied to observe the apoptotic cells. Results: There were different degrees of morphological changes and functions of inhibiting cellactivity and improving apoptosis in each group except control group, which were the most significant in combination group 3. Conclusion: Shenyi Capsule concomitant with endostar can be an optimal choice for the treatment of breast cancer.
       

Methylation Inactivation Mechanism of Parkin Gene mRNA Expression in Nasopharyngeal Carcinoma Hot!

Ni Haifeng, Jiang Bo, Zhou Zhen, Li Yong, Huang Guangwu
Journal of International Translational Medicine, 2014, 2(2): 303-308
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Objective: To investigate the methylation inactivation and the clinical significance of Parkin gene mRNA expression in nasopharyngeal carcinoma (NPC). Methods: The methylation-specific PCR (MSP) and semi-quantitative reverse transcription PCR (RT-PCR) were used to detect methylation and the mRNA expression level of Parkin gene in 54 cases of NPC tissues and 16 cases of normal nasopharyngeal epithelial (NNE) tissues. The mRNA expression of Parkin gene in two NPC cell lines (CNE1 and CNE2) were detected before and after treatment with the methyltransferase inhibitor 5-aza-2-deoxycytidine so as to analyze the effects of Parkin gene methylation and demethylation on Parkin gene mRNA expression and the relationship between Parkin gene mRNA expression and clinical factors. Results: The methylation frequency of Parkin gene in human NPC tissues was 62.96% (34/54), but didn’t happen in any of 16 cases of NNE tissues. The mRNA expression level was (0.3430±0.4947) in 54 cases of NPC tissues and (1.0052±0.4911) in NNE tissues, showing that the mRNA expression level of NPC tissues was significantly down-regulated (P < 0.01). There was significant difference in Parkin mRNA expression of NPC tissues between 34 cases of methylation (0.0942±0.2309) and 20 cases of unmethylation (0.7660±0.4369) (P < 0.01). The Parkin mRNA expression in CNE1 and CNE2 increased after 5-aza-2-deoxycytidine treatment. Parkin gene mRNA expression had no obvious relationship with age, gender, T staging, TNM staging and pathogenic pattern (P > 0.05), but was closely related to lymph node metastasis (P < 0.05). Conclusion: Parkin gene mRNA expression, serving as a cancer suppressor gene in the occurrence and development of NPC, is inactivated and regulated by methylation, which also has a negative correlation with lymph node metastasis and could be considered as the judgment of predictive index of clinical prognosis of NPC.
       

Influence of Radix Astragali, Hirudo, Hirudin and their Compound Medicated Serum on the Growth Cycle and Apoptosis of Glomerular Mesangial Cell in Rats Hot!

Ren Xianzhi, Chen Shasha, Zhai Wensheng
Journal of International Translational Medicine, 2014, 2(2): 309-313
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Objective: To observe the effect of Radix Astragali (RA), hirudo, hirudin and their compound medicated serum on growth cycle and apoptosis of glomerular mesangial cells (GMCs) in rats and their apoptotic morphology. Methods: The prepared cells were randomly divided into control group, hirudo group, hirudin group, RA group and compound group. Flow cytometer was used to detect the growth cycle and apoptosis of GMCs while Wright stain and microscope were applied for the observation of apoptotic cells. Results: RA, hirudo, hirudin and their compound medicated serum could maintain abundant GMCs in gap phase 0/1 (G0/G1) and improve apoptotic rate of GMCs, which had significant differences when compared with control group (P < 0.01). Additionally, they could improve GMCs apoptosis, and differences were significant in hirudo and formula groups when compared with control group (P < 0.01). Conclusion: Hirudo, hirudin, RA and their compound (containing hirudo and RA) can effectively inhibit MC proliferation and promote GMCs apoptosis by stopping GMCs entering phase S of which the efficacy of compound is the best, followed by hirudo.
       

Changes of Proliferation and Apoptosis of K562 Cells after Co-culture with Leukemia Bone Marrow Mesenchymal Stem Cells Hot!

Katja Karjalainen, Carlos E. Bueso-Ramos, Hagop M. Kantarjian
Journal of International Translational Medicine, 2014, 2(2): 314-317
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Objective: To compare the changes of proliferation and apoptosis of K562 cells after co-culture with human leukemia bone marrow mesenchymal stem cells (LMSC). Methods: The prepared cells were randomly divided into SCG group, SCG + 0%FBS group, SCG + 0%FBS group and CCG + 0%FBS group. Cell counting kit-8 (CCK-8) analytic approach was adopted to detect the optical density (OD) of K562 cells in SCG and CCG groups, and the conditions of K562 cell proliferation under different cultured circumstances were compared. Flow cytometer (FCM) was used to detect the changes of K562 cell cycle after co-culture with LMSC, Annexin V/polyimide (PI) fluorescence labeling method to detect the changes of K562 cell apoptosis after co-culture with LMSC and serum starvation. Results: After co-culture with LMSC, the proliferation of K562 cells was markedly inhibited, and OD in CCG group was conspicuously lower than that in SCG group. Flow cytometer (FCM) detection on cell cycles demonstrated that after co-culture with LMSC, the proportion of cells in gap phases 0 ~ 1 (G0 ~ G1) went up notably, whereas that in phase S went down obviously. Besides, the proportion of cells in phases G2 ~ M was on the rise. K562 cell apoptosis in CCG + 0%FBS group was more than in SCG + 10%FBS group, and less than in SCG + 0%FBS group, indicating LMSC had the function of resisting leukemia cell apoptosis. Conclusion: LMSC exerts the effect of inhibiting the proliferation by blocking K562 cell cycles in phases G0 ~ G1, and inhibiting K562 cell apoptosis induced by serum starvation.
       

Expressions of SEL 1L and C-erbB-2 in Gastric Cancer Tissues and Its Clinical Significance Hot!

Xu Guanghui, Li Yu, Li Yan
Journal of International Translational Medicine, 2014, 2(2): 318-321
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Objective: To investigate the expressions and clinical significance of human sel-1-like (SEL 1L) gene and oncogene c-erbB-2 in gastric cancer tissue. Methods: SEL 1L and c-erbB-2 expressions in gastric cancer tissues of 96 patients with gastric cancer were detected by immunohistochemical method. Results: The positive expression rate of SEL 1L gene in gastric cancer tissue was 84.4% (81/96), and its expression was significantly associated with the differentiated degree of gastric cancer tissue (P = 0.001), but had no association with the age, gender, tumor size, infiltration depth, lymphatic metastasis and TNM staging (P > 0.05). The positive expression rate of c-erbB-2 was 70.8% (68/96), and its expression was related to the differentiated degree of gastric cancer tissue, infiltration depth, lymphatic metastasis and TNM staging (P = 0.002, P < 0.05) instead of the age, gender and tumor size (P > 0.05). SEL 1L and c-erbB-2 expressions had a significantly positive correlation (r = 0.568, P < 0.01). Conclusion: Both SEL 1L and c-erbB-2 are highly expressed in gastric cancer tissues and their joint detection can be considered as a biomarker for identifying the high-risk patients with gastric cancer.
       

Expression and its Clinical Significance of CK19 mRNA in Peripheral Blood of Patients with Gastric Cancer Hot!

Zhu Minghui, Zhu Li, Qin Youjuan, Chen Lin
Journal of International Translational Medicine, 2014, 2(2): 322-325
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Objective: To investigate the expression of targeted gene CK19 mRNA in peripheral blood of the patients with gastric cancer and its correlation with biological behaviors of gastric cancer. Methods: CK19 mRNA in peripheral blood of 56 patients with gastric cancer was detected by RT-PCR method. Meanwhile, the peripheral blood of 12 healthy volunteers and 12 gastric cancer tissues were respectively selected as negative and positive controls. The correlation between CK19 mRNA expression in peripheral blood of the patients with gastric cancer and clinical pathological characteristics was analyzed. Results: The positive rate of CK19 mRNA in peripheral blood of 56 patients with gastric cancer was 58.9% (33/56). The expression rate of CK19 mRNA in 12 gastric cancer tissues came up to 100.0% (12/12), whereas CK19 mRNA in peripheral blood of 12 healthy volunteers was expressed negatively. CK19 mRNA expression was significantly related to the clinical staging of gastric cancer and lymphatic metastasis (P < 0.05), but had no association with gender, age, cell differentiation and infiltration depth (P > 0.05). Conclusion: The application of RT-PCR was highly sensitive and specific in detecting the CK19 mRNA in peripheral blood of patients with gastric cancer, in which CK19 mRNA is expected to be a tumor marker for judging the metastasis and recurrence and evaluating the efficacyof gastric cancer.
       

Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer Hot!

Maurie Buehler, Ellie Guardino, Jung Sik Park, Eun Jeong Jang
Journal of International Translational Medicine, 2014, 2(2): 326-330
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Objective: To investigate the clinical application of the detection of HER-2 gene by fluorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene amplification and clinicopathology of breast cancer. Methods: Paraffin-embedded breast infiltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene amplification by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene amplification (P < 0.05). Of the 10 cases with negative estrogen receptor (ER) and progesterone receptor (PR), 7 were with HER-2 gene amplification (P > 0.05). Conclusion: The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene amplification is concerned with axillary nodes metastases.
       

Application of Minimally Invasive Treatment of Locking Compression Plate in Schatzker Ⅰ~Ⅲ Tibial Plateau Fracture Hot!

Zhao Guohui
Journal of International Translational Medicine, 2014, 2(2): 331-335
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Objective: To investigate the clinical effect of minimally invasive treatment of locking compression plate (LCP) in Schatzker Ⅰ~Ⅲ tibial plateau fracture. Methods: Thirty-eight patients with Schatzker Ⅰ~Ⅲ tibial plateau fracture in our hospital were given minimally invasive treatment of LCP, and the artificial bone was transplanted to the depressed bone. Adverse responses, wound healing time and clinical efficacy were observed. Results: All patients were followed-up for 14~ 20 months, and the mean duration was 16 months. Within 1 week after operation, 1 patient suffered from short-term rejection reaction to artificial bone, but he healed after corresponding measures were taken. There were no complications like skin necrosis and externally-exposed steel plate among the patients. In addition, all fractures were recovered, and the recovery time was 2.6 ~ 4.1 months, with the mean duration being 3.4 months. The recovery of knee function was favorable, in which 20 cases were excellent, 14 were good, and 4 were general. The excellent and good rate was 89.5%. Conclusion: Minimally invasive treatment of LCP for Schatzker Ⅰ ~ Ⅲ tibial plateau fracture can reduce the postoperative relocation loss, and has small trauma and stable fixation.
       

Research Progression on Biological Mechanism of Post-stroke Depression Hot!

Lin Facai, Huang Dehong
Journal of International Translational Medicine, 2014, 2(2): 336-340
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The biological mechanism of post-stroke depression (PSD) is still unclear. However, there are two hypothesises including primary endogenous mechanism and reactive mechanism. This study mainly reviewed the biological mechanism of PSD from the aspects of neuroanatomy, neurotransmitter, neuroendocrinology, inflammatory response, neurtrophin and neuropeptide.
       

Research Progression of Anti-HIV Chinese Medicines and Their Natural Active Ingredients Hot!

Chen Jing, Fu Linchun, Li Maoqing, Chen Jiantao
Journal of International Translational Medicine, 2014, 2(2): 341-345
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Objective: To review the research progression of Chinese medicines for anti-human immunodeficiency virus (HIV) and their natural active ingredients at home and abroad so as to provide references for pharmaceutical research and clinical medication. Methods: Abundant representative literatures at home and abroad were classified to introduce the anti-HIV monomers, compounds and natural active ingredients of Chinese medicines. Results: The researches on anti-HIV natural medicines have obtained great achievements, in which medicines with anti-virus and immunity-improving functions have had rapid progression and new-types for anti-virus also have had dramatic development. Conclusion: The progression of anti-HIV Chinese medicines and their active ingredients has favorable prospect.
       

Research Progress of Anti-angiogenesis Drugs in the Treatment of Lymphoma Hot!

Feng Jifeng
Journal of International Translational Medicine, 2014, 2(2): 346-349
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As the targeted drugs come into being in recent years, such as monoclonal antibody, great achievements have been made for the treatment of malignant lymphoma (ML). Rituximab, a monoclonal antibody against CD20 whose effective rate is up to 85% in the first-line treatment of lymphoma, has become the standard first-line treatment of multiple B cell lymphomas. However, how to improve the therapeutic efficacy of B lymphoma and reduce reoccurrence rate in drugresistant patients still need to be further studied. And great importance is increasingly attached to the development of new drugs, especially tumor angiogenesis drugs, in which Bevacizumab and Endostatin are studied as the main representative for the treatment of ML. This paper mainly made a review on Current situation and prospects of anti-angiogenesis drugs for the treatment of lymphoma.
       

Rational Application of Targeted Drugs in the Translational Treatment of Colorectal Liver Metastasis Hot!

Chinese Anti-Cancer Association Colorectal Cancer Specialized Committee
Journal of International Translational Medicine, 2014, 2(2): 350-353
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Complete excision of liver metastases is the only way to potentially heal colorectal liver metastasis. Translational treatment is of great importance for the patients with colorectal liver metastasis in improving the surgical resection rate, prolonging survival time and ameliorating prognosis and its aims are to apply the therapeutic regimen with the highest efficiency to achieve the highest translational resection rate to the greatest extent. In order to further standardize its rational application, improve therapeutic level and prognosis, the experts of Chinese Anti-Cancer Association Colorectal Cancer Specialized Committee proposed their guidance notes for the rational application of targeted drugs in the translational treatment of colorectal liver metastasis to provide references for clinical practice of surgical doctors in our country.
 
 
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