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Journal of International Translational Medicine
Journal of International Translational Medicine
JITM, ISSN 2227-6394
founded in 2012
Editor-in-chief:Feng Jifeng, China
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2013 Vol. 1, No. 3
Published: 30 September 2013

 
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Interventional Mechanism of Tongxieyao Formula on Irritable Bowel Syndrome due to Liver Depression and Spleen Deficiency Hot!

Qian Feng, Bo Ping
Journal of International Translational Medicine, 2013, 1(3): 72-75
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Objective: To investigate the efficacy and interventional mechanism of Tongxieyao Formula (TXYF) on rat model with irritable bowel syndrome (IBS) due to liver depression and spleen deficiency. Methods: 75 newborn SD rats were randomly divided into groups A, B, C, D and E. Except group A, the rats in other groups were treated with senna and strained stress to establish IBS models due to liver depression and spleen deficiency. Groups A and B were fed with intra-gastric normal saline, group C pinaverium bromide, groups D and E TXYF. After treatment, animals were sacrificed to excise colons, and modified toluidine blue staining was applied to observe the changes of colonic mucosal mast cell (MC) count and de-granulation conditions in rats. Then the levels of 5-hydroxytryptamine (5-HT), P substance (SP), calcitonin gene-related peptide (CGRP) in blood samples were detected. Results: The counts of colonic mucosal MC and de-granulation increased significantly in model control group. The levels of 5-HT, SP significantly decreased while CGRP increased in C, D and group Es. Conclusion: TXYF can play its role of improving gastrointestinal mobility and reduce visceral sensitivity so as to treat IBS through reducing the counts of colonic mucosal MC and degranulation as well as the levels of serum 5-HT and plasma SP, and increasing the level of CGRP in IBS model rats.
       

Expression and Significance of HER-2/neu and RECK Proteins in Breast Cancer Tissue Hot!

Shen Bo, Wei Da, Xu Xinyu, Zheng Maqing, Feng Jifeng
Journal of International Translational Medicine, 2013, 1(3): 76-78
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Objective: To investigate the expression and clinical significance of HER-2/neu and RECK proteins in breast cancer tissue. Methods: Seventy-six and 30 samples respectively from breast cancer tissue and normal breast tissue were detected by inmunohistochemical SP assay. The expression of HER-2/neu and RECK proteins in breast cancer tissue and normal breast tissue was observed. Besides, the expression intensity and positive rates of HER-2/neu and RECK proteins at different TNM stagings and differentiated degrees of breast cancer tissue were compared, and the relationship between them was analyzed. Results: The positive rate and intensity of HER-2/neu expression were higher, but those of RECK expression were lower in breast cancer tissue than in normal breast tissue. The positive rates and intensity of HER-2/neu and RECK expression were associated with TNM staging and differentiated tumor tissue, and HER-2/neu and RECK expression in breast cancer tissue had a negative correlation. Conclusion: Overexpression of HER-2/neu and reduced and deleted expression of RECK may be one of the biological mechanisms in the occurrence and progression of breast cancer.
       

Establishment of Adoptive Immunotherapy Transfusion Time of Cytokine-induced Killer Cells Hot!

Wu Changping, Deng Haifeng, Jiang Jingting
Journal of International Translational Medicine, 2013, 1(3): 79-84
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Objective: To investigate the variation of immunophenotype and cytotoxic activity of autologous cytokine-induced killer (CIK) cells in patients with malignant tumors, and explore the best time of adoptive immunotherapy infusion of CIK cells. Methods: Peripheral blood mononuclear cells (PBMC) in 40 patients with malignant tumors were collected and cultivated into CIK cells in vitro by biotechnology under induction of several kinds of cytokines including interferon γ (IFN-γ), recombinant human interleukin 1α (rhIL- 1α), CD3 monoclonal antibody (CD3McAb) and recombinant human interleukin 2 (rhIL-2). Immunophenotypes were dynamically monitored by flow cytometry (FCM), and cytotoxic activity was analyzed by methyl thiazolyl tetrazolium (MTT) method. Results: After induction and expansion at different time, CD3+, CD3+CD8+ and CD3+CD56+ in mononuclear cells (MNC) had an up-regulated tendency. CD3+CD4+ reached the peak on day 7, and then decreased slowly; CD25 reached the peak in earlier period of cultivation (3-7 days), and decreased slowly in 7-14 days, and then decreased rapidly in 14-21 days. Human leukocyte antigen DR (HLA-DR) was on the rise in 0-14 days, and decreased rapidly after reaching the peak on day 14. The cytotoxic activity of mature CIK cells was significantly higher than that of non-activated PBMC, and the difference was statistically significant (P < 0.01). Conclusion: PBMC can be induced into typical CIK cells for about 14 days when CD3+CD56+ cells are at the logarithmic phase. The best time of CIK cell adoptive immunotherapy transfusion for the patients with malignant tumors is on day 14.
       

Efficacy of Icotinib Hydrochloride in the Treatment of Advanced Non-small Cell Lung Cancer Hot!

Ma Xianglei, Tang Yiqun, Kou Yingying, Shi Meiqi
Journal of International Translational Medicine, 2013, 1(3): 85-89
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Objective: To observe and evaluate the efficacy and adverse responses of icotinib hydrochloride in the treatment of advanced non-small cell lung cancer (NSCLC), and analyze the relative factors impacting its efficacy and prognosis. Methods: The clinical data of 260 patients with advanced NSCLC treated with icotinib hydrochloride in Jiangsu Cancer Hospital was retrospectively analyzed. Results: Four weeks after initial administration, 256 patients were evaluable for efficacy except 4 who withdrew the drug due to intolerable adverse responses. Among the 256 patients, there were 0 complete response (CR), 96 partial response (PR, 37.5%), 97 stable disease (SD, 37.9%) and 63 progression disease (PD, 24.6%), with the objective remission rate (ORR) and disease control rate (DCR) being 37.6% and 75.4% respectively. However, in all patients, the median progression-free survival (PFS) was 7 (0.4 ~ 16.3) months, and were 11 (1 ~ 16.3), 6 (0.4 ~ 11.3) and 5 (1 ~ 13.5) months in those treated with first-line, second-line, and ≥third-line treatments, respectively. Conclusion: Icotinib hydrochloride has significant efficiency and better safety for treating advanced NSCLC.
       

Narrow-band Imaging in the Diagnosis of Colorectal Hyper-plastic Lesions Hot!

Zhang Li, Zheng Sijie, Zhang Ming, Yang Lifang, Chen Jinzhen, Li Hai
Journal of International Translational Medicine, 2013, 1(3): 90-94
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Objective: To evaluate the differential diagnostic value of narrow-band imaging (NBI) endoscopy and indigo carmine staining endoscopy in the diagnosis of colorectal hyper-plastic lesions. Methods: A total of 71 lesions form 57 patients were examined with conventional colonoscopy, NBI endoscopy and indigo carmine staining endoscopy to compare and analyze the lesions outlines, pit patterns (PIT) and capillary patterns (CP), so as to distinguish neo-plastic and nonneo- plastic lesions and to compare with the results of pathological diagnosis. Results: The detection rate of conventional endoscopy was 91.5% (65/71), while it was 100% (71/71) under NBI and indigo carmine staining endoscopies, respectively. For the observation of lesion outlines and CP, NBI endoscopy was significantly better than conventional and indigo carmine staining endoscopies, while for the observation of PIT, NBI endoscopy was evidently better than conventional one but similar to indigo carmine staining endoscopy. The diagnostic accuracy rate, sensitivity and specificity of NBI were 91.5% (65/71), 81.8% (18/22) and 95.9% (47/49), which were not statistically significant when compared with indigo carmine staining endoscopy’s 88.7% (63/71), 77.3% (17/22) and 93.9% (46/49), but superior to those under conventional endoscopy. Conclusion: NBI endoscopy is superior to conventional colonoscopy in the diagnosis of colorectal hyper-plastic lesions, especially in the differentiation between neo-plastic and non-neo-plastic lesions. NBI has the advantage of showing the outlines changes of colorectal hyper-plastic lesions, PIT and CP clearly. In addition, it is easy and convenient in operation, so it is also an effective technique to make early diagnosis and has important functions in prevention of CRC.
       

Expression of Uncoupling Protein 2 in Breast Cancer Tissue and Drug-resistant Cells Hot!

Sun Yan, Yuan Yuan, Zhang Lili, Zhu Hong, Hu Sainan
Journal of International Translational Medicine, 2013, 1(3): 95-97
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Objective: To explore the expression of uncoupling protein-2 (UCP2) in clinical breast cancer tissue and drug-resistant cells. Methods: The expression of UCP2 in breast cancer tissue and normal tissue adjacent to carcinoma as well as breast cancer cell MCF-7 and paclitaxel-resistant cell MX-1/T were respectively detected by immunohistochemistry and Western blot. Results: The expression of UCP2 in breast cancer tissue was significantly higher than in normal tissue adjacent to carcinoma, and that in paclitaxel-resistant cell MX-1/T obviously higher than in breast cancer cell MCF-7. Conclusion: UCP2 is highly expressed in breast cancer tissue and drug-resistant cells.
       

Clinical Research of Status Epilepticus: a report of 224 cases Hot!

Tu Qiang, Huang Yi, Xiang Mingqing, Zhu Shaoliang, Ke Chengming, Li Fei
Journal of International Translational Medicine, 2013, 1(3): 98-100
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Objective: To study the clinical efficacy of midazolam in the treatment of 224 patients with status epilepticus (SE). Methods: A total of 224 patients with status epilepticus (SE) admitted in our hospital from October, 2010 to October, 2013 were selected and randomly divided into midazolam group (n = 144) and combination group (tranquillizer + phenobarbital) (n = 80). 0.1 ~ 0.2 mg/ kg of midazolam were slowly given to midazolam group for 5 ~ 10 min while 0.3 ~ 0.5 mg/ kg of diazepam and 5 ~ 10 mg/kg of phenobarbital were intramuscularly injected to patients in combination group. Results: SE’s time was significantly controlled in midazolam group than in combination group, while it was suggested that SE children’s age, etiology, incentives, seizure type, EEG, imaging changes were independent with the short-term efficacy of SE patients (P > 0.05), and the duration of attack, treatment programs and short-term efficacy of SE were correlated (P < 0.05), according to the analysis of age, etiology, incentives, seizure type, duration of attack, laboratory examinations, the relationship between treatment and curative effect. Conclusion: Midazolam is a new BZDs drug containing some special advantages when compared with traditional ones, which is also a favorable anti-epileptic drug with high safety and reliability, rapid onset, simple application and mild toxic responses.
       

EphA2/CD10/Bcl-6/MUM1 contributes to subclassification of diffuse large B cell lymphoma Hot!

Huang Xiaoyin, Wang Jiandong, Sun Qian, Fu Haijin, Guan Xiaoxiang, Wang Jinghua
Journal of International Translational Medicine, 2013, 1(3): 101-106
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Objective: To investigate the clinical and prognostic significance of EphA2 expression in diffuse large B-cell lymphoma (DLBCL). Methods: Immunohistochemistry for EphA2/CD10/Bcl-6/MUM-1 was performed on tissue sections from 51 patients diagnosed with DLBCL, and its correlation with clinicopathologic variables of patients was assessed using Pearson’s χ2 test or Fisher’s exact test when necessary. The survival was analyzed by Kaplan-Meier method. Results: High expression of EphA2 was detected in patients of lower clinical stage (P = 0.001), better international prognostic index (IPI) score (P = 0.020) and germinal center B-cell (GCB) phenotype (P = 0.000). It was also correlated with the expression of CD10 (P = 0.001) and MUM-1 (P = 0.001). Conclusion: EphA2/CD10/MUM1 might contribute to sub-classification of DLBCL.
       

Relationship between Polymorphism of Nicotinic Acetylcholine Receptor Gene CHRNA3 and Susceptibility of Lung Cancer Hot!

Shen Bo, Shi Meiqi, Mei Jinfeng, Hong Zhuan, Cao Guochun, Lu Jianwei, Feng Jifeng
Journal of International Translational Medicine, 2013, 1(3): 107-111
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Objective: To investigate the relationship between polymorphism of nicotinic acetylcholine receptor gene CHRNA3 and susceptibility of lung cancer. Methods: Sixty hundred patients with lung cancer and 600 healthy people were respectively selected. TaqMan-MGB probe technique was applied to detect rs3743073 (T > G) genotypes at SNPs site on CHRNA3. The difference of genotype distribution among groups was compared, and its relationship with lung cancer was also investigated. Results: There was statistical significance regarding the distributions of CHRNA3 rs3743073 (T> G) genotype and allele frequencies in patients with lung cancer and healthy people (P < 0.05). By comparison to the patients with TT genotype, the risk of developing lung cancer in patients with TG and GG genotypes was increased 1.68 times (95%CI = 1.30 - 2.19, P < 0.05) and 1.30 times (95%CI = 1.05 - 1.61, P < 0.05), respectively, while the risk of developing lung cancer in patients with rs3743073G (TG and GG) was increased 0.65 times (95%CI = 0.50 - 0.84, P < 0.05) . Further analysis demonstrated that of the patients more than 60 years old, male and smoking, the risk of developing lung cancer in those with rs3743073G mutant genotypes was elevated significantly (P < 0.05). Conclusion: The risk of developing lung cancer in patients with rs3743073G mutant genotypes of CHRNA3 gene is increased markedly, especially in those more than 60 years old, males and smoking ones.
       

CT-guided 125I Radioactive Seed Implantation on Regional Lymph Node Metastasis after Gastrectomy Hot!

Shi Liangrong, Wu Jun, Jiang Jingting, Wu Changping
Journal of International Translational Medicine, 2013, 1(3): 112-118
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Objective: To evaluate the safety and short-term effect of CT-guided 125I radioactive seed implantation on regional lymph node metastasis after gastrectomy. Methods: Twenty-three patients with regional lymph node metastasis after gastrectomy received CT-guided 125I radioactive seed implantation from June, 2007 to July, 2011 in our hospital. The overall activity and amount of radioactive seeds were calculated by simulating source distribution of radioactive seed implantation plan system before operation. 125I seeds were implanted under the guidance of CT. Effective rate was evaluated according to RECIST criterion, 1, 2 and 3 years of survival rates were calculated by life table method, the effect of relative factors on survival was tested by univariate COX model, and the survival differences between subgroups were compared to draw survival curve by log-rank method. Additionally, systemic therapies were given to 20 patients based on fluorouracil drugs. Results: No severe complications was observed in all study subjects with 14 complete remission (60.9 % ), 5 particle remission (21.7 % ) and 4 progressive diseases (17.4 % ), while 1, 2 and 3 years of survival rates as well as median survival time being (87±7) %,(47±11) % , (13±9) % and (22.1±5.1) months, respectively. Univariate COX analysis showed that the maximum diameter of tumor could badly influence the prognosis (χ2 = 9.752, P = 0.002), and the subgroups analysis relieved the significant difference (χ2 = 5.828, P = 0.016, log-rank test) with 3 cm being the cut-off value. Conclusion: CT-guided 125I radioactive seed implantation has high local control rate with small trauma and slight complications.
       

Study on Invasion of Artesunate on Inhibiting Human Colon Cancer Cell SW620 Hot!

Fan Yu, Zhang Youli, Yao Guangtao, Li Yikui
Journal of International Translational Medicine, 2013, 1(3): 119-122
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Objective: To observe the invasive effect of Chinese extraction artesunate on human colon cancer cell SW620 and explore its possible mechanisms. Methods: Colon cancer cell SW620 was managed by different concentrations of artesunate, and soft agar colony-cultivating trial was applied to detect anchorage independent proliferation of cancer cells, Boyden chamber model method to detect the invasive capability of cancer cells and Western blot method to detect the change of intercellular adhesion molecule-1 (ICAM-1) proteins. Results: Artesunate can effectively inhibit malignant proliferation and invasive capability of colon cancer cell SW620, and was dose-dependent (P < 0.01). Artesunate can effectively inhibit the expression of cancer cell ICAM-1 gene proteins, and was time- and concentration-dependant (P <0.01). Conclusion: Artesunate can significantly inhibit the invasion of colon cancer cell SW620, which can be related to down-regulation of ICAM-1 protein level.
       

Characteristics of Ascending, Descending, Floating and Sinking Theory based on the Concept of Translational Medicine and its Clinical Application Hot!

Wang Xiaohui, Zhai Huaqiang, Wang Wei, Wang Xiaoxia, Zhang Xiaojuan, Zhang Tian
Journal of International Translational Medicine, 2013, 1(3): 123-126
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Ascending, descending, floating and sinking theory is the summarization for the effects of drugs on the body. Translational medicine establishes a bi-directional conversion channels between clinics and laboratories. This article mainly introduces the relationship between traditional Chinese medicine (TCM) ascending, descending, floating and sinking theory and the concept of translational medicine, and how to study this theory based on the concept of translational medicine.
       

Research Progression of Cellular Autophagy in Liver System Diseases Hot!

Liu Chunyun, Gong Xiangwen, Xiao Xinfa, Yuan Xiangying
Journal of International Translational Medicine, 2013, 1(3): 127-134
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Autophagy is a basic biological phenomenon widely existed in eukaryotic cells and an important mechanism for cells to adjust to the surrounding environment, prevent invasion of pathogenic micro-organisms and maintain homeostasis, whose activity changes evidently in multiple liver system diseases, suggesting that there is close association between autophagy and the generation and development of liver system diseases. It is also reported that autophagy develops and exerts an important function in many liver-related diseases, such as hepatic carcinoma, non-alcoholic fatty liver disease, alcoholic liver disease, viral liver disease and acute liver injury. Therefore, this study aimed to summarize the relationship between autophagy and multiple liver diseases, hoping to explore the effect of autophagy in liver system diseases and further study the regulative effect of autophagy so as to provide new thoughts for their treatment.
       

Mechanism and Therapeutic Strategies of Ailamode in the Treatment of RA-induced Bone Loss Hot!

Wang Feng
Journal of International Translational Medicine, 2013, 1(3): 135-143
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Rheumatoid arthritis (RA) caused by exceed bone absorption than osteogenesis and the subsequent osteoporosis (bone loss) around joints and in entire body, is the most commonly seen bone disease in clinic, which is induced by inflammatory factors and corticosteroid therapies, while RA-induced bone loss is believed to be associated with the decreased osteogenesis because of the increased bone absorption and low osterix expression by reason of over-expressions of TNF-α, IL-1, Il-6 and RANKL, etc.. Ailamode (ALMD, T-614) is a new anti-RA agent (DMARDs) and a regulator for immunity and bone metabolism. Research showed that T-614 could eliminate bone absorption and up-regulate osterix expression to improve osteogenesis by inhibiting some inflammatory factors (TNF-α, IL-1 and Il-6), so as to reduce bone and joint damages. And several clinical evidences have proved that T-614 is safe in treating RA and has synergistic effect with methotrexate (MTX), which could strengthen the efficacy and decrease bone erosion. Therefore, it is considered to be the most valuable agent in the treatment of RA and RA-induced bone loss at present.
       

Circulating DNA as Potential Biomarker for Cancer Individualized Therapy Hot!

Yu Shaorong, Liu Baorui, Lu Jianwei, Feng Jifeng
Journal of International Translational Medicine, 2013, 1(3): 144-151
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Cancer individualized therapy often requires for gene mutation analysis of tumor tissue. However, tumor tissue is not always available in clinical practice, particularly from patients with refractory and recurrence disease. Even if patients have sufficient tumor tissue for detection, as development of cancer, the gene status and drug sensitivity of tumor tissues could also change. Hence, screening mutations from primary tumor tissues becomes useless, it’s necessary to find a surrogate tumor tissue for individualized gene screening. Circulating DNA is digested rapidly from blood, which could provide real-time information of the released fragment and make the real-time detection possible. Therefore, it’s expected that circulating DNA could be a potential tumor biomarker for cancer individualized therapy. This review focuses on the biology and clinical utility of circulating DNA mainly on gene mutation detection. Besides, its current status and possible direction in this research area is summarized and discussed objectively.
 
 
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