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Journal of International Translational Medicine
Journal of International Translational Medicine
JITM, ISSN 2227-6394
founded in 2012
Editor-in-chief:Feng Jifeng, China
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2014 Vol. 2, No. 3
Published: 01 September 2014

 
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Interpretations of Chemotherapeutic Protocols on Small Cell Lung Cancer Hot!

FENG Ji-feng
Journal of International Translational Medicine, 2014, 2(3): 354-358 | doi:10.11910/2227-6394.2014.02.03.01
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Brief Introduction of NCCN Clinical Practice Guidelines for Adolescent and Young Adult Oncology Hot!

HUANG Xin-en
Journal of International Translational Medicine, 2014, 2(3): 359-362 | doi:10.11910/2227-6394.2014.02.03.02
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Cancer is always a main factor threatening human’s health and life, and its incidence and mortality are gradually increasing in recent years. However, some advances have been made with the unremitting efforts and exploration human made and the improvement is mainly made in cancer treatment of young children and older adults, while little in adolescent and young adult (AYA) patients, who are generally defined as individuals of 15 to 39 years old at the time of initial cancer diagnosis due to many factors. To highlight the issues of this unique population, National Comprehensive Cancer Network (NCCN) absorbs a large amount of information and previous researches and develops a set of clinical practice guidelines. Though the guidelines are more supportive care guidelines than treatment guidelines, they give us the opportunity to learn the latest international developments in AYA treatment and more survival chance for the treatment of AYA patients.
       

Upregulation of Renin-Angiotensin System in Bone Marrow Mesenchymal Stem Cells Under Hypoxia Conditions Hot!

XIAO Rong-rong, GAO Jing-hong, LI Qing-ping
Journal of International Translational Medicine, 2014, 2(3): 363-369 | doi:10.11910/2227-6394.2014.02.03.03
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Objective: To investigate the expressions of AT1-R, AT2-R and angiotensin converting enzyme (ACE) in mesenchymal stem cells (MSCs) under hypoxia and serum deprivation condition.
Methods: Bone MSCs were isolated, cultured and identified by anti-CD29 and anti-CD11b/c with flow cytometry. The ischemic injury model was established by exposing MSCs to hypoxia and serum deprivation (Hypoxia/SD). Cell viability and apoptotic rate were detected by trypan blue staining, CCK8 assays and Annexin V-FITC staining. The mRNA expressions of AT1-R, AT2-R and ACE were determined by Reverse Transcription-PCR and Real-time Quantitative PCR, The expression of AT1-R, AT2-R and ACE protein were measured by Western-blot.
Results: MSCs expressed CD29, but not the CD11b/c. The purity of MSCs employed was up to 97%. The results of trypan blue staining along with CCK8 and Annexin V-FITC staining proved that the injury model induced by Hypoxia/SD was successfully established. MSCs under hypoxia and serum deprivation for 24h induced a rapid increase in mRNA expression of AT1-R, AT2-R and ACE as well as their protein expressions.
Conclusion: The local RAS in MSCs is activated by Hypoxia/SD stimulation and the mRNA and protein expressions of AT1-R, AT2-R and ACE are up-regulated.
       

Inhibiting Effect and Its Mechanism of Ibandronate on the Proliferation of Humanized NSCLC A549 Cells in Vitro Hot!

YAO Qiang, HUA Dong
Journal of International Translational Medicine, 2014, 2(3): 370-374 | doi:10.11910/2227-6394.2014.02.03.04
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Objective: To explore the effect of ibandronate on the proliferation and the expression of human telomerase reverse transcriptase (hTERT) of non-small cell lung cancer (NSCLC) A549 cell line in vitro.
Methods: Methyl thiazolyl tetrazolium (MTT) assay, microscope, flow cytometry (FCM) and semi-quantitative RT-PCR were employed to detect the cell proliferation, cell cycle as well as the morphological change and the expression of hTERT mRNA of A549 cell line.
Results: The data showed that ibandronate could effectively inhibit the proliferation of A549 cell line in time- and concentration-dependent. Under the microscope, the floating cells increased gradually as the drug concentration increasing. FCM detection showed that ibandronate could induce the cell cycle stopped in G0/G1 phase and downregulation expression of hTERT.
Conclusion: Ibandronate can inhibit the proliferation of A549 cell line in vitro, whose mechanism may be associated with cell cycle arrestted in phase G0/G1 and downregulation expression of hTERT.
       

Establishment and Identification of Chinese Hamster Ovary Cell Lines with Stable Expression of Soluble CD40 Ligands#br# Hot!

JIANG Hua-wei, YANG Quan-liang, LIU Yong-ping, LING Yang
Journal of International Translational Medicine, 2014, 2(3): 375-379 | doi:10.11910/2227-6394.2014.02.03.05
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Objective: To establish the Chinese Hamster Ovary (CHO) cell lines with stable expression of soluble CD40 ligands (sCD40L).
Methods: Recombinant plasmid pIRES2-EGFP-sCD40L, enzyme digestion and sequencing identification were obtained by cloning sCD40L coding sequences into eukaryotic expression vector pIRES2-EGFP from carrier pDC316-sCD40 containing sCD40L. CHO cells were transfected by electroporation, followed by screening of resistant clones with G418, after which monoclones were obtained by limited dilution assay and multiply cultured. Flow cytometer and reverted fluorescence microscope were applied to observe the expression of green fluorescent protein, while sCD40L expression was detected by polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) from aspects of deoxyribose nucleic acid (DNA), messenger ribonucleic acid (mRNA) and protein, respectively. CHO-sCD40L was cultured together with MDA-MB-231 cells to compare the expression changes of surface molecule fatty acid synthase (Fas) by flow cytometer and observe the apoptosis of MDA-MB-231 cells after Fas activated antibodies (CH-11) were added 24 h later.
Results: Plasmid pIRES2-EGFP-sCD40L was successfully established, and cell lines with stable expression of sCD40L were obtained with cloned culture after CHO cell transfection, which was named as B11. Flow cytometer and reverted fluorescence microscope showed >90% expression of green fluorescent protein, while PCR, RT-PCR and ELISA suggested integration of sCD40L genes into cell genome DNA, transcription of sCD40L mRNA and sCD40L protein expression being (4.5±2.1) ng/mL in the supernatant of cell culture, respectively. After co-culture of B11 and MDA-MB-231 cells, the surface Fas expression of MDA-MB-231 cells was increased from (3±1.02) % to (34.8±8.75)%, while the apoptosis rate 24 h after addition of CH11 from (5.4±1.32)% to (20.7±5.24)%, and the differences were statistically significant when compared with those in control group (P<0.01).
Conclusion: CHO cell lines with stable expression of sCD40L are successfully obtained and provide useful appliances for the application of CD40/CD40L pathways in tumor immnuotherapy.
       

Effect of Evodiamine on Inducing Apoptosis of Human Gastric Cancer Cell Line SGC-7901 in Vitro Hot!

LIU Shao-ping, QIAN Wen-jun, MAO Wei-dong
Journal of International Translational Medicine, 2014, 2(3): 380-384 | doi:10.11910/2227-6394.2014.02.03.06
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Objective: To explore the proliferation inhibition and apoptosis-inducing effect of evodiamine in human gastric cancer SGC-7901 cells.
Methods: After 48 or 24 h exposure to different concentrations of evodiamine, cell proliferation was analyzed using tetrazolium blue (MTT) assay while apoptosis and cell-cycle phase distribution using flow cytometry.
Results: In 0.01~30.00 μg/mL range of concentrations, evodiamine inhibited the proliferation of SGC-7901 cells in dose-dependent manner, and the overall mean IC50 was (3.79±0.16) μg/mL; the apoptosis rate was increased from 3.4% to 7.0%, 13.8% and 36.3% at concentrations of 0, 0.5, 1.5 and 30 μg/mL of evodiamine, respectively; the percentage of cells accumulated in G2/M phase was increased from 17.26% to 98.92% in the cells treated with evodiamine for 24 h in 0.01~30.00 μg/mL range of concentrations.
Conclusion: Evodiamine can inhibit the proliferation, induce apoptosis in human gastric cancer cell line SGC-7901 in vitro and arrest the cell cycle at the G2/M phase.
       

Suppressive Effect of Icaritin on Angiogenesis and Its Mechanisms Hot!

ZHANG Da
Journal of International Translational Medicine, 2014, 2(3): 385-388 | doi:10.11910/2227-6394.2014.02.03.07
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Objective: To explore the suppressive effect of icaritin on angiogenesis and its mechanisms.
Methods: After 48 or 24 h exposure to different concentrations of icaritin, cell proliferation was analyzed using tetrazolium blue (MTT) assay, the migration ability of Human umbilical vein endothelial cells (HUVEC) was tested in a Transwell Chamber and tube formation ability of HUVEC was determined by tube formation assay in vitro.
Results: Icaritin inhibited the proliferation of HUVEC in dose-dependent manner; Tubes with high density formed in control group while treated with icaritin in 15~60 μg/mL range of concentrations, the number of tubes decreased and the lumen was incomplete. After treatment with icaritin, migration cells were significantly less than those in control group. Tube formation and migration ability was inhibited in dose-dependent manner with a correlation coefficient of -0.934 and -0.933, respectively.
Conclusion: Icaritin can effectively inhibit the angiogenesis of HUVEC in vitro and its mechanism may be related to the inhibition of proliferation, migration and tube formation.
       

 Clinical Application of Total Knee Arthroplasty on Patients with Advanced Knee Osteoarthritis#br# Hot!

WU Zhi-sen, ZHENG Chen-xiao, QI Liang, CHANG Shang-yi
Journal of International Translational Medicine, 2014, 2(3): 389-392 | doi:10.11910/2227-6394.2014.02.03.08
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Objective: To investigate the clinical value of total knee arthroplasty (TKA) on patients with advanced knee osteoarthritis.
Methods: The clinical data and efficacy of 26 patients with advanced knee osteoarthritis (26 knees) who were given TKA in our department from June 2012 to May 2013 were retrospectively observed and analyzed. The knee function scores before operation and after follow up were evaluated according to American HSS scoring standard.
Results: At the end of follow up, of the 26 patients, 18 were excellent, 6 were good and 2 were not bad in knee function and mobility without sense of pain, which was regarded to be associated with the poor enthusiasm in knee function training, and the total rate of excellent and good was 92.3%.
Conclusion: TKA has significant clinical value and favorable efficacy on patients with advanced knee osteoarthritis.
       

Analysis of Positive Human Immunodeficiency Virus (1+2) Antibodies in Preliminary Screening: A Report of 394 Cases Hot!

NI Fang, LIU Yan-yan, MA Cai-yun, WU Zhi-qi, XU Hua-guo, JIANG Li
Journal of International Translational Medicine, 2014, 2(3): 393-397 | doi:10.11910/2227-6394.2014.02.03.09
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Objective: To comprehend the characteristics of patients with positive human immunodeficiency virus (HIV) (1+2) antibodies in the preliminary screening so as to provide basis for local HIV screening and prevention.
Methods: Enzyme-linked immunosorbent assay (ELISA) was used to detect serum HIV (1+2) antibodies in the preliminary screening, after which the positive serum was sent to acquired immune deficiency syndrome (AIDS) confirmatory laboratory to be confirmed with western blotting method. Clinical data of patients with positive HIV antibodies in the preliminary screening in the outpatients and inpatients from 2006 to 2013 were collected and analyzed.
Results: A total of 394 patients with positive serum HIV antibodies were screened initially, in which 214 were confirmed positive HIV, 13 were not certain and another 167 were negative. Patients with positive serum HIV antibodies in the preliminary screening were increased from 9 cases in 2006 to 94 cases in 2013, in which those confirmed with positive HIV increased from 5 to 49. Patients with positive serum HIV antibodies in the preliminary screening and those confirmed increased annually. In addition, patients confirmed with positive serum HIV antibodies were mainly males and aged 20~49 years, distributing in Departments of Infections, Respiratory, Dermatology, Hematology and Emergency, whereas those confirmed with negative HIV were mainly females and aged >20 years, distributing in Departments of Hematology, Maternity and Emergency as well as Reproductive Center.
Conclusion: HIV infection is in low level with characteristics of annually increasing infection rate, male-orientated and wide-spread distribution in variuos departments, etc., knowing which will help guide the clinical practices and carry out the local HIV screening and prevention by relevant departments.
       

Expressions of Ezrin and CD44v6 in Gastric Cancer Hot!

DAI Shi-min
Journal of International Translational Medicine, 2014, 2(3): 398-402 | doi:10.11910/2227-6394.2014.02.03.10
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Objective: To explore the expressions of cytoskeletal protein Ezrin and cell adhesion molecule CD44v6 in gastric cancer patients and their significances.
Methods: Expressions of Ezrin and CD44v6 in paraffin embedded samples from 80 patients with gastric cancer (pathological group) and samples from 30 people with normal gastric mucosa (normal group) were detected by immunohistochemical test and their relationships with clinicopathological factors were analyzed.
Results: The expressions of Ezrin in pathological and normal groups were 65.0% and 30.0%, while those of CD44v6 were 62.5% and 0%, respectively. The expression degree of Ezrin was associated with the differentiation degrees of gastric cancer (P<0.05) and was significantly related with clinical stages, lymph node metastasis and distant metastasis (P<0.01). However, the expression of CD44v6 had no connection with the differentiation degrees of gastric cancer (P>0.05) but was closely correlated with clinical stages, lymph node metastasis and distant metastasis (P<0.01). In addition, there was obviously positive association between the expressions of Ezrin and CD44v6 (P<0.01).
Conclusion: The high expressions of Ezrin and CD44v6 are closely associated with clinical stages, lymph node metastasis and distant metastasis, and their combined detection can be used as important predicative index for the metastasis and prognosis of patients with gastric cancer.
       

Expressions of 11beta-Hydroxysteroid Dehydrogenase Types 1 and 2 in Human Colorectal Cancer Hot!

WANG Ji-rong, XU Hong-xia
Journal of International Translational Medicine, 2014, 2(3): 403-407 | doi:10.11910/2227-6394.2014.02.03.11
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Objiective: To observe the expressions of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. 
Methods: Neoplastic tissues and autologous non-neoplastic tissues were taken from 12 patients with colorectal cancer immediately after the operations. The mRNA expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and 11beta-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western- blot assay. 
Results: Both isoforms of 11β-HSD expressed in the colon adenocarcinoma, but their expressions were not identical in neoplastic and non-neoplastic tissues. There was a significant decrease of 11β-HSD2 mRNA abundance and protein abundance in neoplastic tissue. 11β-HSD1 protein and mRNA abundance were not significantly different from those in control tissue. 
Conclusions: Neoplastic transformation is associated with the decreases of 11β-HSD2 mRNA and protein.
       

The Expression of Ezrin and Its Significance in Non-small Cell Lung Cancer Hot!

MA Lan, WU Hao, SUN Jing, LIU Lian-ke
Journal of International Translational Medicine, 2014, 2(3): 408-412 | doi:10.11910/2227-6394.2014.02.03.12
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Objective: To explore the expression of ezrin and its significance in non-small cell lung cancer (NSCLC).
Methods: The expression of ezrin in 81 NSCLC patients was examined by immunohistochemical staining Envision method, and its relationship with the clinopathologic features and other indexes was analyzed.
Results: The high expression rate of ezrin in NSCLC was 59.26% (48/81) and significantly correlated with lymph node metastasis (P<0.05), but not associated with gender, histologic subtype, differentiation, TNM stages or smoking. The K-M survival analysis demonstrated that patients with the over-expression of ezrin were obviously poorer than those with low-expression of ezrin (P<0.05).
Conclusion: The expression of ezrin may be associated with metastasis of NSCLC, can be as an important marker for evaluating the prognosis of NSCLC patients.
       

Terminal Cancer: Malignant Spinal Cord Compression and Full Code Status Hot!

Yaseen Ali, Amila M. Parekh, Rahul K. Rao, Mirza R. Baig
Journal of International Translational Medicine, 2014, 2(3): 413-415 | doi:10.11910/2227-6394.2014.02.03.13
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Background: Malignant spinal cord compression has significantly increased hospitalization costs and even with best approach in treatment the disease course remains relatively stable with dire outcomes.
Case presentation: The patient was an 80 years old male with the past medical history of hypertension, stroke with chronic right sided weakness, recently diagnosed with non-squamous cell lung carcinoma stage T4N0Mx presently undergoing chemotherapy as outpatient with carboplatin and taxol presented to the emergency room with the chief complaint of right leg pain with weakness and chest pain for 1~2 days. On d 4 of the admission patient complained of chest pain again and a CT angiogram was ordered as part of the work up for chest pain based on high probability for a pulmonary embolus per “Wells Score”. The CT angiogram revealed a large soft tissue mass centered at T5 vertebral body and probable spinal canal invasion.
Conclusion: A more favorable outcome requires the input of both a surgeon and a radiation oncologist to find the most effective approach depending on the area involved and the extent of the lesion, and patient’s choice of treatment always must be respected as well. Despite aggressive treatment patient did not respond well and was deteriorating. Options were discussed with the patient, including the futility of care and lack of response. Patient opted to return home with hospice care and was subsequently discharged home with family.
       

 

Hot!
JIAO Lei, XU An-hua, FENG Chao, QIU Qian-qian, TANG Qi-ling, LIU Xiao-huan1
Journal of International Translational Medicine, 2014, 2(3): 416-420 | doi:10.11910/2227-6394.2014.02.03.14
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Ebola virus disease (EVD) is an acute hemorrhagic infectious disease caused by ebola virus, with high infectivity and fatality rate. At present, it mainly occurs in areas of Central Africa and West Africa and no effective vaccine and antiviral drugs are available for the clinical treatment.
       

  Review on Different Action Mechanisms of PDGF-BB on Glomerular Mesangial Cell Proliferation#br# Hot!

CHEN Sha-sha, REN Xian-zhi
Journal of International Translational Medicine, 2014, 2(3): 421-425 | doi:10.11910/2227-6394.2014.02.03.15
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Platelet-derived growth factor BB (PDGF-BB) and PDGF receptor-β (PDGFR) play critical roles in proliferation and envolving into MsPGN of mesangial cells in embryonic development. The mechanisms can be demonstrated mainly from several aspects such as promoting the proliferation of glomerular mesangial cells (GMC), stimulating the GMC expression of extracellular matrix components and synergism with other growth factors like transform growth factor-β (TGF-β) and so on. As in the pathological development of mesangial proliferative lesions, GMCs is not only the victim of kidney damage but also the direct participants. GMCs can secrete excessive PDGF - BB and other growth factors which could react to GMCs, leading to malignant progress of kidney damage. Therefore, inhibiting GMCs over expression of PDGF-BB and blocking the effect of PDGF-BB on promoting proliferation and ECM accumulation is undoubtedly an effective way to delay kidney damage.

       

Exerting the Role of Journal of International Translational Medicine in Translational Medicine Hot!

YE Zhen-hua, DU Fu-rong, YANG Xue, WU Yin-ping, YI Zi, CHEN Chong
Journal of International Translational Medicine, 2014, 2(3): 426-428 | doi:10.11910/2227-6394.2014.02.03.16
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As a new branch of medicine with unlimited prospect, Translational Medicine has been rapidly developed over the past decades and gained global attention. Translational Medicine can be vividly described as “bench to bedside (B2B)” model which focuses on the integration of multiple aspects, especially the basic medicine and clinical medicine, and has a great potential in treating many severe diseases and improving human health. Medical journals are carriers for medical information and play a great role in the development of medicines, and Journal of International Translational Medicine (JITM) is just a journal emerging at the right moment and specialized in translational medicine. With professionalized attitude and internationalized requirements, JITM is committed to promote the development of Translational Medicine.
 
 
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