Effect of Fluvastatin on Oxidation-reduction Function and ICAM-1 Expression in Pocine Carotid Artery Endothelial Cells Dealt with Hypoxia/Reoxygenation
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A Protective Effect of Ischemic Preconditioning on the Rat Lung Ischemia-Reperfusion Injury
Detection of Apoptotic Inhibitor Gene Survivin in Peripheral Blood of Patients with Esophageal Cancer by Real-time Fluorescence Quantitative PCR and its Clinical significance Hot!
Pro-apoptosis Effect of Survivin T34A Mutant on Cancer Cells in Vitro and Vivo Hot!
Objective: To explore the pro-apoptosis effect of Survivin T34A mutant on cancer cells in vitro and vivo.
Methods: After highly-metastasized breast cancer cells (4T1 cells) were transfected with Survivin T34A plasmid and wild survivin plasmid, cell proliferation was analyzed using tetrazolium blue (MTT) assay and apoptosis was detected with flow cytometry. In animal experiments, mice were vaccinated subcutaneously with 4T1 cells and treated with T34A plasmid and wild survivin plasmid. The tumor volume and weight, wet weight of the lung, number of pulmonary metastasis nodule were measured. H&E staining and TUNEL detection of tumor apoptosis were performed after mice were executed.
Results: The cell survival rate was significantly decreased (P＜0.01) and apoptotic rate increased (P＜0.01) after treatment with Survivin T34A plasmid in vitro. In vivo, 4 days after treatment, tumor volume was significantly smaller, mean tumor weight and mean wet weight of the lung were obviously lighter, and pulmonary metastasis nodule was evidently fewer (P＜0.05). The apoptotic cells and large areas of necrosis were observed, and apoptotic index was increased (P＜0.05).
Conclusion: Survivin T34A can induce the apoptosis of 4T1 cells with specificity and may become a new approach to breast cancer.
Study on 64-layer Spiral Computed Tomography Perfusion Imaging in Patients with Fatty Liver Hot!
Influence of Decitabine on Demethylation of P15INK4B Gene and the Growth and Apoptosis of Burkitt Lymphoma Raji Cells
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Interpretation of Guideline for Diagnosis and Treatment of Dengue Fever Hot!