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Journal of International Translational Medicine
Journal of International Translational Medicine
JITM, ISSN 2227-6394
founded in 2012
Editor-in-chief:Feng Jifeng, China
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2015 Vol. 3, No. 1
Published: 15 March 2015

 
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Protective Effect of Angiopoietin-1 on the Blood Brain Barrier after Focal Cerebral IschemiaReperfusion Injury in Rats

GAO Xuan, LI Hong-liang, LI Yong-qiu, LU Lin, ZHAO Ming-yan
Journal of International Translational Medicine, 2015, 3(1): 1-5 | doi:10.11910/2227-6394.2015.03.01.01
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  Objective: To observe the protective effect of angiopoietin-1 (Ang-1) on the blood brain barrier (BBB) after focal cerebral ischemia-reperfusion injury (CIRI) in rats. Methods: Forty male healthy Wister rats were selected. The focal CIRI models were established using middle cerebral artery occlusion and 30 rats were divided into sham-operation group (n=10), normal saline group (NS) group (n=10) and Ang-1 treatment group (n=10). The rats were killed 48 h after reperfusion, and brain water content, BBB permeability, cerebral infarction volume and neurological severity scores in rats were detected respectively. 
Results: 48 h after reperfusion, both the brain water content and Evans blue (EB) content in Ang-1 treatment group were significantly lower than in NS control [(68.69±4.46) % vs. (79.08±4.12) %, P<0.001; (98.60±10.56) μg/g vs. (379.90±21.64) μg/g, P<0.001], and there was no statistical significance by comparison to those in sham-operation group [(65.37±3.28) %; (89.62±8.65) μg/g, P>0.05)]. The cerebral infarction volume in Ang-1 treatment group was markedly smaller than in NS group [(0.119±0.022) cm3 vs. (0.407±0.036) cm3, P<0.001], and no significant difference was presented when compared with sham-operation group [(0.104±0.011) cm3, P>0.05]. Besides, the neurological severity scores in Ang-1 treatment group were notably lower than in NS group [(1.83±0.29) points vs. (2.87±0.34) points, P<0.001], but higher than in sham-operation group [(0.79±0.11) points, P<0.001]. 
Conclusion: Ang-1 can decrease BBB permeability after focal CIRI in rats, so it has a protective effect on CIRI.

       

Total Flavonoids from Mimosa Pudica Protects Carbon Tetrachloride -Induced Acute Liver Injury in Mice

QIU Zhen-qin, CAI Lei, CHEN Da-shuai
Journal of International Translational Medicine, 2015, 3(1): 6-10 | doi:10.11910/2227-6394.2015.03.01.02
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Objective: To observe the protective effect of total flavonoids from Mimosa pudica on carbon tetrachloride (CCl4)-induced acute liver injury in mice.
Methods: CCl4-induced acute liver injury model in mice was established. The activity of ALT and AST, the content of serum albumin (Alb) and total antioxidant capacity (T-AOC) were determined. The content of malondiadehyde (MDA) was measured and the activity of superoxide dismutase (SOD) was determined. The histopathological changes of liver were observed.
Results: Compared with CCl4 modle group, each dose group of total flavonouida from Mimosa pudica couldreduced the activity of ALT and AST in mice obviously (P<0.01), indicating they had remarkably protective effect on CCl4-induced acute liver injury in mice. high and middle dose groups of total flavonouida from Mimosa pudica couldincrease the content of Alb in mice (P<0.01). Each dose group of total flavonouida from Mimosa pudica could enhance the level of T-AOC (P<0.01). each dose group of total flavonouida from Mimosa pudica could lower the content of liver homogenate MDA but enhance the activity of SOD in a dose-depended manner (P<0.01).    
Conclusion: Total flavones from Mimosa Pudica have obvious protective effect on CCl4-induced acute liver injury in mice.
       

Expression of PPARγ, p27 and Ki67 in Cervical Cancer and its Clinical Significance

LI Peng-li, TAN Hai-zhi
Journal of International Translational Medicine, 2015, 3(1): 11-17 | doi:10.11910/2227-6394.2015.03.01.03
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Objective: To investigate the expression of peroxisome proliferation-activated receptor γ
(PPARγ), p27 and Ki67 in cervical cancer and its clinical significance.
Methods: The expression of PPARγ, p27 and Ki67 in the tissues of 42 patients with cervical
cancer, 28 with cervical intraepithelial neoplasia (CIN) and 12 with normal cervix was detected
using immunohistochemistry.
Results:The positive rate of PPARγ protein in cervical cancer tissue (76.2%) was significantly
higher than in CIN (53.6%) and normal cervical tissue (8.3%) (P<0.05 orP<0.01), which was
also evidently higher in CIN than in normal cervical tissue (P<0.05). The positive rate of p27
protein in cervical cancer tissue (31.0%) was significantly lower than in CIN (57.1%) and normal
cervical tissue (83.3%) (P<0.05 or P<0.01), and that in CIN had a markedly lower tendency
compared with normal cervical tissue (P<0.05). The positive rate of Ki67 protein in cervical
cancer tissue (100.0%) was apparently higher than in CIN (85.7%) and normal cervical tissue
(33.3%) (P<0.05 or P<0.01), which was also markedly higher in CIN than in normal cervical
tissue (P<0.01). The expression of PPARγ, p27 and Ki67 proteins was not associated with the
clinicopathological features of patients, including the age, histological types, pathological grading
and clinical staging (P>0.05).
Conclusion: Abnormal expression of PPARγ, p27 and Ki67 may play important roles in
occurrence and progression of cervical cancer, and hence, joint detection of PPARγ, p27 and Ki67
can be used to diagnose early CIN and cervical cancer.
       

Clinical Analysis of Pulmonary Lipiodol Embolism in Patients with Hepatic Carcinoma after Transcatheter Arterial Chemoembolization Hot!

JIANG Wen-jin&, WANG Li-gang&, SONG Xue-peng&, ZHENG Yan-bo*, LIU Xiao-gang, SUN Bo-lin
Journal of International Translational Medicine, 2015, 3(1): 18-22 | doi:doi:10.11910/2227-6394.2015.03.01.04
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Objective:To explore the clinical manifestations, therapeutic methods and preventive measures of
pulmonary lipiodol embolism (PLE) induced by transcatheter arterial chemoembolization (TACE)
so as to improve the cognition and management of PLE.
Methods:A total of 2 613 patients with hepatic cancer without history of pulmonary disease
who were treated with TACE in our hospital from Sept., 2004 to Mar., 2013 were selected. The
clinical manifestations, therapeutic methods and preventing measures of the 9 patients who
were accompanied with PLE were observed to analyze the pre-operative hepatic computed
tomography (CT) and chest X-ray, intra-operative contrast examination, dosage of lipiodol
and chemotherapeutic drugs, clinical manifestation and therapeutic progression as well as the
postoperative follow-up.
Results: Nine patients accompanied by PLE had different-severity cough, hemoptysis and
progressive dyspnea, and chest X-ray and/or CT showed flaky high-density radiography. After
treated with oxygen inhalation, bronchus expansion and inflammation alleviation, 8 patients were
improved but 1 died. Of the 8 patients, 2 were given ventilator to assist breath, and the clinical
symptoms of 8 patients disappeared within 3~15 d. The re-examined chest X-ray showed normal
after 20~60 d follow-up observation. Additionally, 6 patients were with nidus diameter ≥10
cm, 6 with hepatic artery-vein fistula and 7 with lipiodol dosage ≥20 mL.
Conclusion: PLE often occurs in patients with giant hepatic carcinoma accompanied by hepatic
artery-vein fistula, whose lipiodol dosage is ≥20 mL. Accurate and correct management during
operation can effectively reduce the development of PLE.
       

Influence of Hyperlipidemia on the Proliferation and Apoptosis of Myocardial Cells in Rabbits

Huse Kinscherf1, Cynthia C. Haudenschild2, Christian C. Chobanian3
Journal of International Translational Medicine, 2015, 3(1): 23-27 | doi:10.11910/2227-6394.2015.03.01.05
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Objective: To establish hyperlipemia models in rabbits fed with high fat diet, observe the changes
of serum lipid level, and to explore the effect of hyperlipidemia on the proliferation and apoptosis
of myocardial cells in rabbits.
Methods: New Zealand white male special rabbits were randomly divided into 2 groups: normal
diet group and high-fat-fed group, 10 cases in each group. The rabbits in normal group were fed
with ordinary feed while the rabbits in the latter group were fed with high fat diet. The levels of
serum TC, TG, HDL-C and LDL-C were detected at 0, 4th, 7th, 10th, 13thweeks for 2 groups. At the
end of the 13th week, the pathological changes of myocardial tissues were detected by HE staining,
and the proliferation and apoptosis of cardiomyocyte were tested by FCM.
Results:After given high fat diet, the level of serum TC, TG, HDL-C and LDL-C in high-fat-fed
diet group were significantly increased than in the normal diet group from the 4th week to the 13th
week (P<0.01). The construction of cardiomyocyte in the atherogenic diet group had obviously
morphological changes. The distribution of G0/G1 phase in high-fat-fed increased significantly
than that in the normal diet group (P<0.01), but S phase and proliferation index (PI) in decreased
significantly than that in the normal diet group (P<0.01). The apoptosis rate in both groups had
no statistical difference (P>0.05).
Conclusion: Hyperlipidemia can induce the pathological changes of cardiomyocyte in rabbits,
inhibit the proliferation of cardiomyocyte, but has no effect on the apoptosis of cardiomycyte in
rabbits.
       

Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP Hot!

CAI Lei1, RAO Xiao-hui2, SU Qi3, QIN Jia-sheng1, CAI Li-quan1, HONG He1, WANG Kang-hua1, AI Zhi-guo2, ZHANG Sheng2
Journal of International Translational Medicine, 2015, 3(1): 28-32 | doi:10.11910/2227-6394.2015.03.01.06
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Objective: To explore the applicative value of serum Golgi protein (GP73) and alpha-proteinvariant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).
Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked
immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo’s chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP. 
Results: The levels of serum GP73 and AFP-L3(%) in PHC group were significantly higher than the other 2 groups (P<0.01) and the level of serum GP73 and AFP-L3 (%) in Non-PHC group were higher than those in healthy group (P<0.01). ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(%) in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78 μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0 vs. 71.7 and 60.0, P<0.05) but the specificity was similar between single detection and joint detection (P>0.05). The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was significant differences in negative predictive value (P<0.05) but no difference in positive predictive value (P>0.05). The overall response rate of joint detection of GP73 and AFP-L3 was higher than single detection of them (P<0.05).
Conclusion: single detection of GP73 and AFP-L3 (%) were similar in the sensitivity and specificity in diagnosis of PHC patients with low concentration of AFP, which can be auxiliary diagnosis for PHC patients and joint detection of them is more reliable to diagnose PHC.
       

Expression of MGMT, hMLH1 and XRCC1 in Gastric Cancer Tissue and Their Clinical Significance

LI Xiao-feng1, LIU Ai-yong2, LI Jia3
Journal of International Translational Medicine, 2015, 3(1): 33-38 | doi:10.11910/2227-6394.2015.03.01.07
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Objective: To study the expression and their correlation of repair gene MGMT, hMLH1 and XRCC1 and to explore the relationship between their expressions and clinicopathologic features of gastric cancer.
Methods: Immunohistochemical SP method was used to detect the expression of repair gene MGMT, hMLH1 and XRCC1 in 52 gastric cancer specimens. The relationship between MGMT, hMLH1, XRCC1 and clinicopathological features and the correlation between MGMT and hMLH1 and XRCC1 were analyzed.
Results:The positive rates of MGMT, hMLH1 and XRCC1 proteins were 75.0% (39/52), 63.7% (33/52) and 76.8% (40/52), respectively. The expression MGMT protein was positively correlated to the expression of hM-LH1 (r=0.498, P<0.05), but no obvious correlation to the expression of XRCC1 (P>0.05). The MGMT expression was associated with histological pattern, lymphatic metastasis, distant metastasis and postoperative recurrence (P<0.05, orP<0.01) but not associated with gender, age, invasive depth and tumor staging (P>0.05). hMLH1 expression was related to histological pattern, lymphatic metastasis and postoperative recurrence (P<0.05, or P<0.01), but not related to gender, age, tumor staging, invasive depth and distant metastasis (P>0.05). XRCC1 expression was related to histological pattern, lymphatic metastasis, tumor staging and postoperative recurrence (P<0.05, or P<0.01), but not related to gender, age, invasive depth and distant metastasis (P>0.05).
Conclusion: MGMT, hMLH1 and XRCC1 expression is closely related to the occurrence of gastric cancer and has the effect against the development of gastric cancer.
       

Values of Seven Tumor Markers in Identification and Diagnosis of Esophageal Carcinoma Accompanied by Neuroendocrine Differentiation Hot!

LIU Lian-ke*, SHAO Ming-wen, MA Lan, SUN Jing, GUAN Dan, SHU Yongqian
Journal of International Translational Medicine, 2015, 3(1): 39-43 | doi:10.11910/2227-6394.2015.03.01.08
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Objective: To explore the values of seven tumor markers in the identification and diagnosis of esophageal carcinoma accompanied by neuroendocrine differentiation (E-NED).
Methods:A total of 378 patients diagnosed as low differentiation of esophageal carcinoma in The First Affiliated Hospital with Nanjing Medical University from Jan., 2008 to Dec., 2013 were selected, in which there were 349 with esophageal carcinoma with no neuroendocrine differentiation (E-NNED, E-NNED group) and 29 with E-NED (E-NED group). The levels of seven tumor markers including synaptophysin (Syn), Chromogranin A (CgA), neuron-specific enolase (NSE), neural cell adhesion molecule (CD56), protein gene product 9.5 (PGP9.5), secretagogue (SCGN) and  thyroid transcription factor-1 (TTF-1) of both groups were detected
with histoimmunochemical method and the influences of the single and combined detection of above indexes on E-NED patients were analyzed.
Results: Except TTF-1, expressions of Syn, CgA, NSE, CD56, PGP9.5 and SCGN in E-NED group were evidently higher than those in E-NNED group and the differences were significant (P<0.05). Syn, PGP9.5, CD56 and SCGN had higher sensitivity, positive predicative value (PPV) and diagnostic accurate rate (DAR) but with low specificity whereas NES was low in both sensitivity and specificity and TTF-1 higher in specificity and lower in sensitivity. Compared with detection of single index, the sensitivity, PPV and DAR of combined detections of Syn+CD56, Syn+PGP9.5 or Syn+SCGN were markedly higher than those of Syn+CgA, and the
differences were significant (P<0.05).
Conclusion: PGP9.5 and SCGN can be used as neuroendocrine markers for the pathological diagnosis of E-NED and Syn+CD56, Syn+PGP9.5 and Syn+SCGN can all be used as combined detection.
       

Meig’s Syndrome: A Triad of Pleural Effusion, Abdominal Ascites, and Benign Ovarian Fibroma Hot!

Yaseen Ali, Amila M. Parekh, Rahul K. Rao, Taseen Ali, Linda S. Schneider, Jordan Garvey, Mirza R. Baig
Journal of International Translational Medicine, 2015, 3(1): 44-46 | doi:10.11910/2227-6394.2015.03.01.09
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Background:Meig’s syndrome is a rare syndrome characterized by a triad of recurrent pleural effusions, ascites, and the finding of a benign ovarian fibroma on diagnostic imaging and histopathological evaluation. Patients can present with any of the constellation of symptoms attributing to the disease state. With pleural effusions they can present with shortness of breath, chest pressure, dyspnea on exertion; symptoms that can be confused with the exacerbation of congestive heart failure. Ascites can present with abdominal tenseness, pain, bloating, cramping, constipation, and elevated liver enzymes. The finding of a benign ovarian fibroma is found only during diagnostic imaging and histopathological evaluation. Case report:The patients was an 85-year-old female with a recent history of coronary artery bypass graft surgery for her severe coronary artery disease presented with the chief complaint of generalized malaise, abdominal pain, constipation of few days. She was initially scheduled to have her second therapeutic thoracentesis for her recurrent pleural effusion as an outpatient procedure but complained of the former symptoms and was admitted for observation and treatment of her abdominal symptoms. Her recurrent pleural effusions were initially attributed to the complications of her coronary artery bypass graft surgery for her severe coronary artery disease. During the admission and evaluation she was diagnosed with Meig’s syndrome. She underwent a left oophorectomy with total abdominal hysterectomy that led to the resolution of all her symptoms. Conclusion:Meig’s syndrome is a rare syndrome characterized by the triad of recurrent pleural effusions, ascites, and the finding of a benign ovarian fibroma. The diagnosis and knowledge of this syndrome holds the key to its treatment. The treatment generally involves the resection of the ovarian fibroma. After the resection of the ovarian fibroma patients recover from the inconvenient pleural effusions and ascites with no recurrence or future surveillance needed.
       

Advances in Translational Medicine of Breast Cancer: From Bench to Bedside Hot!

HUANG Xin-en
Journal of International Translational Medicine, 2015, 3(1): 47-52 | doi:10.11910/2227-6394.2015.03.01.10
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Breast cancer is one of the most common malignant tumors in women, and its incidence increases year by year. With the rapid development of human genomics, proteomics and bioinformatics, the basic and clinical results of breast cancer have emerged in endlessly. Translational medicine is a hot field of international medicine, biological interdisciplinary science and many research funding projects at present, committed to establishing an academic exchange platform for global scientific researchers. In this study, the translational medicine of breast cancer was summarized and reviewed from the following aspects, including molecular markers related to breast cancer, molecular typing, individualized and molecular targeted therapies, relationship between molecular biology and imagiology of breast cancer.
       

Advances of Chinese and Western Medicine on Rheumatoid Arthritis Hot!

HE Na-na1, WU Zhi-sen2, XING Zhen-long3, ZHANG Zhi-gang4, SUN Shi-xuan5, LIN Fa-cai1*
Journal of International Translational Medicine, 2015, 3(1): 53-58 | doi:10.11910/2227-6394.2015.03.01.11
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Rheumatoid arthritis (RA) is a kind of chronic general immunological disease with primary clinical characteristic being joint synovitis, and no specific treatment has been found at present. In China, its morbidity is in an increasing trend, and also shows a trend of getting younger. RA is complicated in symptoms and chronic disease course, leading to joint ankylosis, deformity and even disability, which seriously threaten patients’ psychological and physical health as well as the quality of life (QOL). RA, which belongs to “ Bi” symdrome, is termed as “arthroncus of knee” or “severe and migratory arthralgia” in traditional Chinese medicine (TCM), for which TCM focuses on expelling wind to remove cold, tonifying qi to activate blood and dispelling dampness to smooth collaterals while the western medicines mainly based on the principles of anti-inflammation, analgesia and inhibiting autoimmune response. However, both Chinese and western medicines have certain advantages in treating RA. Therefore, this study primarily reviewed the advances of Chinese and western medicines in RA treatment in the hope of providing theoretical basis for the clinical treatment of RA.
       

Analysis on Current Status and Plights of Translational Medicine

ZHAO Jin-wen, QIAN Feng
Journal of International Translational Medicine, 2015, 3(1): 59-66 | doi:10.11910/2227-6394.2015.03.01.12
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Translational medicine, which is a new concept appearing in the field of international medical health in the past three or four years, forms a discipline of the system medicine (including systematic pathology, diagnosis and comprehensive treatment as well as pharmacology, etc) synchronizing with personalized medicine and predictable medicine. It is a branch of medical research trying to build a better bridge between the basic studies and the clinical medical treatment. It can promote the predicative, prognostic and personalized medicines by the evaluation and application of molecular markers, the individual therapies based on molecular subtypes and the evaluation and predication of therapeutic responses and prognosis. And its purpose is to build a direct connection between basic research and clinical practice so as to transform the knowledge and achievements of basic research into new therapeutic methods in clinic, which can be used to serve the clinic and solve clinical problems. Nevertheless, the study on translational medicine is limited, and there are few review articles about the progress of translational medicine in English. With the development of innovative and new technology, the therapeutic methods become more accurate and diverse, and the translational medicine will make outstanding advances and provide more space for the future treatment of various diseases so as to better serve the clinical practices and the patients. In addition, except the outstanding achievements, there are still multiple plights in the translational medicine research. Therefore, this study aimed to review the current status and the present problems in translational medicine, hoping to expand the study outcomes in China to the whole world.
       

Interpretation of Clinical Practice Guidelines for Diagnosis and Treatment of Hodgkin’s Lymphoma Hot!

FENG Ji-feng
Journal of International Translational Medicine, 2015, 3(1): 67-71 | doi:10.11910/2227-6394.2015.03.01.13
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